What is MicroSort®?

MicroSort® is a scientifically proven sperms sorting technology that sorts sperms into X sperms and Y sperms, which improves the chances that the baby you conceive will be of the desired gender. Semen samples typically contain equal amounts of sperm carrying the Y chromosome (which will produce a boy) and sperm carrying the X chromosome (which will produce a girl).

What method is MicroSort® technology based on?

MicroSort® is a sperm separation method based on the difference in the amount of genetic material in sperm. Sperm carrying an X chromosome has approximately 2.8% more DNA material than sperm carrying a Y chromosome. The flow cytometer is able to measure the quantitative difference of genetic material in sperm cells, and sort into X- sperms and Y- sperms one by one.

Who ought to consider utilizing MicroSort®?

MicroSort® is available for couples who have certain genetic diseases that are gender-linked or gender-hereditary diseases such as haemophilia or Duchenne Muscular Dystrophy and who are looking to balance their families.

How does MicroSort® sort sperms?

During the MicroSort® process, the sperm sample is washed to remove seminal liquid and nonmotile cells. After the washing, the sample is stained with a special fluorescent material that attaches to the DNA contained in the sperm.

The stained sperm cells are analyzed one by one by a flow cytometer, in which cells pass through a laser to make the stain attach to the DNA fluoresce. The sperm containing the X chromosome (which have more DNA and therefore more stain) will shine brighter than the sperm containing the Y chromosome. The flow cytometer uses a special software to identify and sort sperm cells for enrichment of sperm samples (enriched X or Y chromosome sperm)

How to utilize the sorted sperms?

The samples processed by MicroSort® can be used in a wide variety of artificial reproduction techniques, which range from Intrauterine Insemination (IUI) to a full In Vitro Fertilization (IVF) cycle — that can include intracytoplasmic sperm injection (ICSI) and Preimplantation Genetic Diagnosis/Screening (PGD/PGS), among other procedures.

What are the required minimum cells?

The minimum of motile sperms required by IUI is 100 million(100)x106)whereas its counterpart for IVF or ICSI is 70 million (70×106)。These values refer to the total number of motile sperm cells regardless of their capacity. In a frozen sample, these quotas refer to the total amount of motile sperms after being thawed.  If the semen sample of a patient doesn’t reach to the minimum of required motile sperms, the patient shall freeze more sperm samples to achieve it. If there is any semen analysis, these sperm samples could be transmitted to doctors who would inform you whether you are appropriate for MicroSort participation or not, or you have to consider various options.

How many sperms can be sorted by each procedure?

MicroSort® aims to recycle sperms as many as possible with an average amount of 100,000-300,000 and with an average motility of 75% after being classified. These figures may vary from original quality of semen samples and the result is within the range of previously mentioned the minimum sperms.  Based on the place where assisted pregnancy is conducted (local or international), the sorted samples could be frozen in multiple vials for future usage (but only applied for IVF or ICSI).

Can samples be frozen, preserved and transmitted back and forth from MicroSort laboratories?

Yes, semen samples can be frozen and preserved, then shipped to MicroSort laboratories for sorting out. Samples after being sorted out can be frozen for preservation and shipped to the designated fertility center(recommend to be applied only for IVF or ICSI). The applicable amount of motile sperms will be reduced by frozen preservation and thaw process, which is why we suggest that multi semen samples should be frozen to achieve at least 200 million motile sperms in total.

Can frozen and preserved samples be applicable for Intrauterine Insemination (IUI)?

50%-60% of sperms don’t survive after being thawed and they have a comparatively lower motility. Thus, sperms after being frozen and preserved are recommended only for IVF or ICSI procedure.

How is the effect of sorted sperm?

Purity of sorted sperms is shown in the following table:

Semen Samples

X-sperm Y-sperm
Unsorted category 50% 50%
Sorted category 88%(X-sorted) 74%(Y-sorted)

MicroSort® does not guarantee a baby of the desired gender, but the technology is scientifically proven to significantly increase the likelihood of conceiving a baby of the desired gender. In terms of the number of babies of the desired gender born, MicroSort® has been 93% effective for girls and 82% effective for boys.

What are the requirements before conducting sorting procedure?

MicroSort® laboratories require two different tests which shall prove that you are an appropriate candidate at least a few days ahead of the sorting procedure.

  1. Semen analysis: Semen analysis in advance to assure whether semen samples contain enough sperm cells for the sorting procedure or not, which is extremely significant. After 3-4 days abstinence, conducting semen analysis is to confirm the quantity of sperms, their motility and progress ability.
  2. Blood test: This blood test includes HIV I and II antibody, Hepatitis B antibody and Hepatitis B surface antigen, Hepatitis C antibody and Syphilis.